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Literature summary extracted from

  • Sakamoto, Y.; Taguchi, T.; Honke, K.; Korekane, H.; Watanabe, H.; Tano, Y.; Dohmae, N.; Takio, K.; Horii, A.; Taniguchi, N.
    Molecular cloning and expression of cDNA encoding chicken UDP-N-acetyl-D-glucosamine (GlcNAc):GlcNAcbeta1-6(GlcNAcbeta1-2)-Manalpha1-R[GlcNAc to Man]beta1,4N-acetylglucosaminyltransferase VI (2000), J. Biol. Chem., 275, 36029-36034.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.201 cloning from cDNA library, DNA sequence determination, functional expression in COS-1 cells Gallus gallus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.201 additional information
-
 type II transmembrane protein, primary structure, amino acid sequence Gallus gallus
2.4.1.201 60000
-
 x * 60000, reducing SDS-PAGE Gallus gallus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.201 UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2)-beta-D-mannosyl-R Gallus gallus biosynthesis of pentaantennary asparagine-linked N-glycans UDP + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2-beta)-(N-acetyl-D-glucosaminyl-1,4)-beta-D-mannosyl-R
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.201 Gallus gallus Q9DGD1
-
-
2.4.1.201 no activity in african green monkey
-
 COS-1 cells
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.4.1.201 glycoprotein 2 potential N-glycosylation sites Gallus gallus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.201 colon
-
 Gallus gallus
-
2.4.1.201 lung
-
 Gallus gallus
-
2.4.1.201 oviduct
-
 Gallus gallus
-
2.4.1.201 spleen
-
 Gallus gallus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.201 0.00000045
-
 recombinant enzyme in COS-1 cells Gallus gallus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.201 UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2)-beta-D-mannosyl-R R represents the remainder of the N-oligosaccharide core of asparagine-linked glycopeptides, (+/-)-fucose, minimum structure requirement: N-acetyl-D-glucosaminyl-beta-1,6-(N-acetylglucosaminyl-beta-1,2)-alpha-mannosyl-R Gallus gallus UDP + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2-beta)-(N-acetyl-D-glucosaminyl-1,4)-beta-D-mannosyl-R
-
 ?
2.4.1.201 UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2)-beta-D-mannosyl-R biosynthesis of pentaantennary asparagine-linked N-glycans Gallus gallus UDP + N-acetyl-D-glucosaminyl-1,6-beta-D-(N-acetylglucosaminyl-1,2-beta)-(N-acetyl-D-glucosaminyl-1,4)-beta-D-mannosyl-R
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.201 ? x * 60000, reducing SDS-PAGE Gallus gallus

Synonyms

EC Number Synonyms Comment Organism
2.4.1.201 GnT VI
-
 Gallus gallus
2.4.1.201 UDP-N-acetyl-D-glucosamine (GlcNAc):GlcNAcbeta1-6(GlcNAcbeta1-2)-Manalpha1-R[GlcNAc to Man]beta1,4N-acetylglucosaminyltransferase VI
-
 Gallus gallus